humanized anti-Tac (HAT) binding peptide - Other Categories

Affinity chromatography and protein purification are more successful with highly selective ligands such as short peptides. Phage libraries have been utilised to identify novel peptides for target proteins. IgG1 monoclonal antibody is traditionally purified using protein A but is not ideal due to cost and methodology. EPIHRSTLTALL was found via phage library screening as the most selective ligand possible IgG1, and also highly stable. It binds to the constant region of IgG1 known as humanized anti-Tac (HAT). HAT is a humanized monoclonal antibody against the low-affinity p55 subunit of the interleukin IL-2 receptor.