Peptide design guidelines

How to design peptides

Peptide design can play a crucial role from a technical perspective. Depending on amino acids’s nature and position, a peptide can be difficult to synthesize or purify, difficult to solubilize or poorly stable over the time. Here are few basics tips we can disclose. As each peptide is different, feel free to get in touch with us.

  • Avoid peptide with more than 50% hydrophobic amino acids content (see section peptide solubility guidelines). Mutate by polar aa such as Gly or Pro
  • Try to have charged residues in the sequence (R, K, D, E)
  • Basic peptides have to be preferred (positive net charge)
  • Avoid Q (Gln) in N-terminal extremity, it tends to cyclize during the chemical production
  • Avoid -DG- sequences
  • Shorter is better. Short sequences <20 amino acids are more easy to synthesize
  • Avoid oxidation-sensitive amino acids (Met, Cys, Trp). Met can eventually be replaced by Noreleucin, cystein by serine, tryptophane by lysine
  • Highly acid peptides can be capped in N-ter by acetyl group to gain a negative charge
  • Highly basic peptide can be amidated in C-ter to gain a positive charge

Custom peptide synthesis service

Visit our custom peptide synthesis webpage and peptide modification webpage to discover what SB-PEPTIDE can offer or contact us to discuss about your project.