TFA removal service

Choose the appropriate salt form of your peptide to better suit your needs – TFA removal

SB-PEPTIDE offers trifluoroacetic acid TFA removal service.
During solid phase synthesis process, TFA is used to release synthesized peptides from resin. And also to deprotect the lateral chains of amino acids. TFA may also be used during the reversed-phase HPLC purification of peptides. Due to resin cleavage and purification conditions, the resulting peptides contains TFA salts. Anionic TFA counterions interact with the free amine group at the N-terminus as well as side chains of positively charged residues (lysine, histidine, and arginine).

TFA is a strong acid and the presence of TFA-salts counterions in the peptide preparation is detrimental for biological material. TFA ultimately can affect the accuracy and reproducibility of in cellulo and in vivo studies. Peptides supplied by SB-PEPTIDE are lyophilized in the TFA format. If TFA-salts counterions are problematic for your experiment, TFA salt exchange by another salt form, such as acetate or hydrochloride is required. For biological applications, replacement of TFA salt counterions with acetate is recommended. Acetic acid (pKa = 4.5) is a weaker acid than both trifluoroacetic acid (pKa ≈ 0) and hydrochloric acid (pKa = −7). Consequently, the exchange of TFA salts for acetate counterions is easier.

Peptide TFA removal

The removal of TFA salts counterions is a challenging procedure, since the ion pairing is strong. This service is available as an option, thereby additional charge applies. This includes the required raw material and the peptide loss that occurs during the salt exchange process.

In addition, it is worth noting that bound TFA salts can alter the apparent mass of a peptide as determined by weighing. For this reason, the quantity of our peptides is given in net content rather than Gross Weight generally reported.

Company history

SB-PEPTIDE is a French company specialized in peptide synthesis, peptide engineering and peptide analysis. Peptides are 100% made in France in the company’s facilities located in the Grenoble area (French Alps).

SB-PEPTIDE is a subdivisary of the company Smartox Biotechnology, worldwide renowned for its expertise in animal venom research. Smartox Biotechnology is a pre-clinical stage company developing drugs inspired from animal venom peptides. The company collaborates with largest pharmaceutical company and is developing a proprietary pipeline of drugs. During more than 10 years + academic background, Smartox Biotechnology has developed an extensive expertise in peptide synthesis, engineering and analysis which is now accessible by the life-science community through SB-PEPTIDE.

Managing team

Rémy Béroud_Peptide synthesis

Rémy Béroud, co-founder & President, Smartox Biotechnology and SB-PEPTIDE.

Missions

SB-PEPTIDE offers premium, flexible and reliable services, focused on the satisfaction of its clients. Peptide synthesis services from the Smartox group have been cited in various scientific publications and patents (see section Citations & achievements). SB-PEPTIDE is acting as a CMO/CRO under its own name or as an OEM and supplies public, pharmaceutical and biotech laboratories, agroscience & cosmetic companies as well as CROs or distributors. The company has already successfully synthesized peptides up to 120 amino acids with adequate quantity and purity, and has performed peptide folding up to 5 disulfide bonds and double labeling.

Get a Quote