mBD3 peptide – Potent antimicrobial peptide
Mouse Beta-Defensin 3 (mBD3) peptide, a homolog of human beta-defensin 2 (hBD2), is a cysteine-rich cationic antimicrobial peptide of 41 amino acids which belongs to the defensins family.
Defensin peptides are cationic peptides originally produced in various organs. They are involved in the protection against different kinds of pathogens like bacteria, fungi, and several viruses and play an important role in inflammation and wound repair.
mBD3 peptide showed antimicrobial activity against Gram-negative bacteria S.Aureus and S.pyogenes and against Gram-positive bacteria like certain strains of P.Aeruginosa (MIC of 8 µg/mL) and E.coli (MIC of 16 µg/mL). An antimicrobial activity has also been demonstrated against C.Albicans.
The in vivo and in vitro efficiency of mBD3 peptide against viruses like influenza was also shown. mBD3 peptide can be interesting for therapeutic applications.
|Sequence : KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K|
|MW : 4 617.57 Da (C195H328N68O50S6)|
|Purity : > 95%|
|Disulfide Bonds : Cys8-Cys37, Cys15-Cys30, Cys20-Cys38|
|Counter-Ion : TFA Salts (see option TFA removal)|
|Delivery format : Freeze dried in propylene 2mL microtubes|
|Other names : Murine Beta Defensin 3|
|Peptide Solubility Guideline|
|Bulk peptide quantities available|
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1- Jiang Y, Yang D, Li W, Wang B, Jiang Z, Li M. Antivir Chem Chemother. (2012)
2- Bals R, Wang X, Meegalla RL, et al. Infect Immun. (1999)
Mouse Beta-Defensin 3 Is an Inducible Antimicrobial Peptide Expressed in the Epithelia of Multiple Organs
BACKGROUND: One component of host defense at mucosal surfaces is epithelium-derived peptides with antimicrobial activity called defensins.
OBJECTIVE: We describe in this report the isolation and characterization of a murine homologue of human beta-defensin 2 (hBD-2) called mouse beta-defensin 3 (mBD-3).
METHOD/RESULTS: The predicted amino acid sequence shows the hallmark features of other known epithelial defensins, including the ordered array of six cysteine residues. Analysis of a genomic clone of mBD-3 revealed two exons separated by a 1.7-kb intron. The mBD-3 gene is localized at the proximal portion of chromosome 8, the site where genes for mouse alpha- and beta-defensins are found. Under basal condition, mBD-3 transcripts were detected at low levels in epithelial cells of surface organs, such as the intestine and lung. After instillation of Pseudomonas aeruginosa PAO1 into mouse airways, mBD-3-specific mRNA was upregulated significantly not only in large airways but also in the small bowel and liver. Recombinant mBD-3 peptide, produced from a baculovirus expression system, showed antimicrobial activity against P. aeruginosa PAO1 (MIC of 8 micrograms/ml) and Escherichia coli D31 (MIC of 16 micrograms/ml) in a salt-dependent manner.
CONCLUSION: This study demonstrates that a murine homologue of hBD-2 is present in the respiratory system and other mucosal surfaces. These similarities between murine and human host defense apparatus provide further impetus to evaluate the mouse as a model for studying the human innate host defense system.