Globin Reduction PNA Set

Human globin mRNA - PCR Blocker

Globin Reduction PNA is a non-enzymatic solution designed to selectively reduce alpha and beta globin mRNA from total RNA extracted from whole blood. It works as a clamp during reverse transcription, binding specifically to globin transcripts and preventing their amplification, which allows more efficient detection of non-globin mRNA targets. Since globin mRNA represents a large proportion of total mRNA in blood (often over 70%), its removal significantly improves the sensitivity and accuracy of downstream gene expression analysis.

Main characteristics

Globin Reduction PNA is designed to limit the impact of globin mRNA during whole blood transcriptome analysis. It works by binding specifically to globin transcripts during reverse transcription, blocking their conversion into cDNA and preventing their amplification in PCR. Since globin mRNA makes up a large portion of total RNA in blood, this reduction step improves the detection of non-globin genes, leading to more sensitive and accurate gene expression analysis.. A terminal glycine is added to the PNA structure to improve flexibility and reduce steric hindrance, which can help optimize binding efficiency without affecting specificity.

Applications

Reduces globin mRNA interference in whole blood RNA samples
Improves sensitivity of gene expression analysis in blood transcriptomics
Enhances RNA-seq data quality by increasing non-globin transcript detection
Supports biomarker discovery in blood-based studies
Enables more accurate profiling of low-abundance transcripts
Applicable to clinical, diagnostic, and research workflows using whole blood RNA

Globin Reduction PNA Set consists of four PNA oligonucleotides specifically designed to target alpha and beta globin mRNA, and is effective for both human and mouse samples:

Globin- Reduction PNA SB339: taacggtatttggag-G
Globin-Reduction PNA SB340: gtagttggacttagg-G
Globin-Reduction PNA SB341: gcccttcataatatc-G
Globin- Reduction PNA SB342: atccagatgctcaag-G

Why Choose the Complete Globin Reduction PNA Set?

In whole blood transcriptomics, hemoglobin is produced by multiple distinct genes that code for different polypeptide chains, primarily alpha and beta globin. Using a single PNA sequence would only inhibit one specific transcript, allowing the remaining globin mRNA types to dominate the sample and consume the majority of your sequencing resources.

By utilizing the complete Globin Reduction PNA Set, you ensure a comprehensive suppression of all major globin variants. This collective approach is essential to effectively lower the total globin cDNA concentration. By neutralizing the entire family of these abundant transcripts simultaneously, you reallocate sequencing depth toward the rest of the transcriptome. This process is the only way to achieve the sensitivity required to identify rare biomarkers and low-abundance nuclear genes that are otherwise obscured by the high concentration of hemoglobin signals.

Technical specification

	Sequency :	PNA SB339: taacggtatttggag-G PNA SB340: gtagttggacttagg-G PNA SB341: gcccttcataatatc-G PNA SB342: atccagatgctcaag-G
	MW :	PNA SB339: 4214.97 g/mol PNA SB340: 4230.97 g/mol PNA SB341: 4054.88 g/mol PNA SB342: 4128.93 g/mol
	Purity :	> 95%
	Counter-Ion :	TFA Salts
	Delivery format :	Lyophilized

Price

Product	Size	Price €	Price $
SB339-342 - 25nmol	25 nmol	354	438
SB339-342- 50 nmol	50 nmol	582	699

Product	Size	Price €	Price $
SB Globin Reduction PNA Set - 25nmol	25 nmol	1021	1226
SB Globin Reduction PNA Set- 50 nmol	50 nmol	1631	1957

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References

Clinical Biochemistry Volume 40, Issue 7, April 2007, Pages 499-502

Objectives:

Whole-blood RNA for microarray analysis is easily accessible but contains a large proportion of globin mRNA that interferes with the accurate assessment of other genes. This study investigated the biological significance of genes whose expression was unmasked by globin mRNA reduction in peripheral blood.

Design and methods:

Samples were collected from healthy subjects using the PAXgene Blood RNA System, and globin mRNA was depleted using GLOBINclear. Genes exhibiting consistent changes in expression on Affymetrix HU133A 2.0 arrays were characterized in three main areas of gene ontology — molecular function, biological process, and cellular component.

Results:

Globin reduction permitted detection of 2652 ± 395 additional genes per assay. Genes unmasked by globin reduction include low abundance transcripts that function primarily as molecular binding proteins and catalytic enzymes in biological processes including transcription, replication, and intracellular transport and signalling. Protein products of these genes are preferentially associated with membranes and the nucleus.

Conclusions:

Additional genes detectable only after globin reduction in whole-blood RNA function in a variety of biological processes that may be important to diverse fields of study.

2016 Aug 12:6:31584. doi: 10.1038/srep31584.

Globin mRNA reduction for whole-blood transcriptome sequencing

The transcriptome analysis of whole-blood RNA by sequencing holds promise for the identification and tracking of biomarkers; however, the high globin mRNA (gmRNA) content of erythrocytes hampers whole-blood and buffy coat analyses. We introduce a novel gmRNA locking assay (GlobinLock, GL) as a robust and simple gmRNA reduction tool to preserve RNA quality, save time and cost. GL consists of a pair of gmRNA-specific oligonucleotides in RNA initial denaturation buffer that is effective immediately after RNA denaturation and adds only ten minutes of incubation to the whole cDNA synthesis procedure when compared to non-blood RNA analysis. We show that GL is fully effective not only for human samples but also for mouse and rat, and so far incompletely studied cow, dog and zebrafish.

29 Jan 2008 - https://doi.org/10.2217/17520363.2.1.11

Comparison of Globin RNA Processing Methods for Genome-Wide Transcriptome Analysis from Whole Blood

Aims: Whole blood is likely to become the prime tissue to detect biomarkers using gene expression analyses. In this study, we assessed whether whole-blood or globin-reduced RNA provides the most robust and sensitive results to detect small gene expression changes, for example, in response to hormone therapy (HT) exposure. Material & methods: Each sample (n = 12) was processed according to three different protocols: no globin reduction, globin reduction using peptide nucleic acids (PNAs) and globin reduction using magnetic beads in the GlobinClear™ kit from Ambion. Results: Both globin reduction approaches using Ambion kit and PNAs were efficient at reducing globin RNA. However, globin reduction using Ambion kit also lowered the remaining cRNA. Samples processed with PNAs gave an intermediary profile closest to the no globin reduction group, with a slight increase in sensitivity of transcript detection and decrease in variability, but a loss of reproducibility. Conclusions: Globin RNA processing method in blood transcriptome analyses should be optimized for each microarray platform to be used. In our study, globin reduction was not found to be beneficial using the Applied Biosystems microarray system (AB1700).

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