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(CAG)5-FAM-Ahx

CAG Repeat PNA Probe

(CAG)5-FAM-Ahx probe is a synthetic PNA probe designed to recognize CAG trinucleotide repeat sequences and is labeled with FAM, enabling green fluorescence detection in FISH experiments. These repeats are associated with disorders such as Myotonic Dystrophy Type 1 (DM1) and Huntington’s disease, making the probe useful in both research and diagnostic applications. Its PNA backbone ensures high binding specificity and strong resistance to enzymatic degradation. The FAM fluorophore provides a bright and clear signal in the green spectrum, allowing accurate visualization and high-resolution mapping of repeat regions.

Main characteristics

(CAG)5-FAM-Ahx probe specifically recognizes (CAG)n repeat sequences associated with repeat expansion disorders and is labeled with FAM (Ex/Em = 495/520 nm), enabling clear green fluorescence detection under microscopy. Its PNA backbone ensures strong binding specificity, high stability, and minimal background signal. An Ahx spacer (6-aminohexanoic acid, CAS: 60-32-2) is included to improve accessibility of the binding region, while a terminal glycine can be added to enhance molecular flexibility and support efficient hybridization. The probe performs reliably in both metaphase and interphase cells and is suitable for single-probe or multiplex FISH applications.

Applications
  • Detection of CAG repeat expansions using FISH techniques
  • Support for studies and diagnosis of DM1 and Huntington’s disease
  • Cytogenetic analysis of trinucleotide repeat–related disorders
  • Visualization and mapping of repeat regions on chromosomes
  • Co-localization studies with additional genetic or repeat markers
  • Research on genome instability and neurodegenerative diseases

 

Technical specification

KD20 peptide Sequency : FAM-Ahx-cagcagcagcagcag-G
KD20 peptide MW : 4214.62 g/mol
KD20 peptide Purity : > 95%
KD20 peptide Counter-Ion : TFA Salts
Peptide library synthesis KD20 peptide Delivery format : Lyophilized

Price

 

Product Size Price €
 Price $
SB345 - 25nmol 25 nmol 465 558
SB345 - 50 nmol 50 nmol 682 819

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References

March 14, 1995 92 (6) 1901-1905 https://doi.org/10.1073/pnas.92.6.1901

Isolation of active genes containing CAG repeats by DNA strand invasion by a peptide nucleic acid.

Abstract

An amplification of tandem CAG trinucleotide sequences in DNA due to errors in DNA replication is involved in at least four hereditary neurodegenerative diseases. The CAG triplet repeats when translated into protein give rise to tracts of glutamine residues, which are a prominent feature of many transcription factors, including the TATA-binding protein of transcription factor TFIID. We have used a biotin-labeled, complementary peptide nucleic acid (PNA) to invade the CAG repeats in intact chromatin and then employed a method for the selective isolation of transcriptionally active chromatin restriction fragments containing the PNA.DNA hybrids. The PNA-containing chromatin fragments were captured on streptavidin-agarose magnetic beads and shown to contain all the CAG.PNA hybrids of the active chromatin fraction. DNA hybridization experiments using a DNA probe specific for unique sequences downstream of the CAG-tandem repeats confirmed that the PNA.DNA hybrids contained the transcribed gene for the TATA-binding protein. In contrast, no hybridization signal was detected with a DNA probe specific for the c-myc protooncogene, which is amplified and transcriptionally active in COLO 320DM cells but lacks CAG tandem repeats.

DOI: 10.1039/D2RA00230B (Paper) RSC Adv., 2022, 12, 7757-7761

PNA microprobe for label-free detection of expanded trinucleotide repeats

Abstract

We present a PNA microprobe sensing platform to detect trinucleotide repeat mutation by electrochemical impedance spectroscopy. The microprobe platform discriminated Huntington's disease-associated CAG repeats in cell-derived total RNA with S/N 1:3. This sensitive, label-free, and PCR-free detection strategy may be employed in the future to develop biosensing platforms for the detection of a plethora of repeat expansion disorders.

https://doi.org/10.1016/S1359-6446(03)02611-4

The impact of nucleic acid secondary structure on PNA hybridization

Abstract

Hybridization of oligonucleotides and their analogues to complementary DNA or RNA sequences is complicated by the presence of secondary and tertiary structure in the target. In particular, folding of the target nucleic acid imposes substantial thermodynamic penalties to hybridization. Slower kinetics for hybridization can also be observed, relative to an unstructured target. The development of high affinity oligonucleotide analogues such as peptide nucleic acid (PNA) can compensate for the thermodynamic and kinetic barriers to hybridization. Examples of structured targets successfully hybridized by PNA oligomers include DNA duplexes, DNA hairpins, DNA quadruplexes and an RNA hairpin embedded within a mRNA.

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